goat anti-mouse serum albumin (polyclonal) (R&D Systems)

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R&D Systems goat anti-mouse serum albumin (polyclonal)

Goat Anti Mouse Serum Albumin (Polyclonal), supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti-mouse+serum+albumin+(polyclonal)/mouse+cxcl10+ip+10+crg+2/pmc08127948-51-0-6
Average 90 stars, based on 1 article reviews

goat anti-mouse serum albumin (polyclonal) - by Bioz Stars, 2026-10

90/100 stars

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1) Product Images from "A stromal progenitor and ILC2 niche promotes muscle eosinophilia and fibrosis-associated gene expression"

Article Title: A stromal progenitor and ILC2 niche promotes muscle eosinophilia and fibrosis-associated gene expression

Journal: Cell reports

doi: 10.1016/j.celrep.2021.108997

Figure Legend Snippet:

Techniques Used: Recombinant, Saline, Blocking Assay, Plasmid Preparation, Lysis, cDNA Synthesis, Staining, Avidin-Biotin Assay, Software

Saline:

Article Title: KREMEN1 variants associated with ectodermal dysplasia impair complex formation of KREMEN1 with DKK1 and LRP6 and attenuate WNT3A response
Article Snippet: For relative gene expression, the 493 comparative cycle threshold (ΔΔCT) values were calculated based on the data generated with the 494 QuantStudio Design & Analysis software v1.5.1 (Thermo Fisher Scientific). .. 495 496 Antibodies and reagents 497 Primary antibody and dilution used: monoclonal rabbit anti-DKK1 (CellSignaling; #48367; diluted 498 1:500 in 2.5% milk in Tris-buffered saline with Tween 20 (TBS-T)), polyclonal rabbit anti-KREMEN1 499 antibody (Invitrogen; PA5-34520; diluted 1:500 in 2.5% BSA in TBS-T or 1:500/1:1,000 in 2.5% milk in 500 TBS-T), polyclonal rabbit anti-KREMEN1 antibody (Invitrogen; PA5-87955; diluted 1:500 in 2.5% milk 501 or BSA in TBS-T), polyclonal rabbit anti-KREMEN1 antibody (Invitrogen; PA5-14549; diluted 1:500 in 502 2.5% milk or BSA in TBS-T), and polyclonal goat anti-KREMEN1 antibody (R&D Systems; AF2127; 503 diluted 1:1,000 in 2.5% milk or BSA in TBS-T). .. 504 Fluorescent dye or horseradish peroxidase (HRP)-linked secondary antibodies and dilutions 505 used: monoclonal anti-Flag M2-Peroxidase antibody (Sigma; #A8592; diluted 1:30,000 in 2.5% milk in 506 TBS-T), anti-HA-Peroxidase antibody (Roche; #12013819001; diluted 1:20,000 in 2.5% milk in TBS-T), 507 mouse anti-goat IgG-HRP (Santa Cruz Biotechnology; sc-2354; 1:10,000), hFAB Rhodamine anti-508 GAPDH antibody (Bio-Rad; #12004167; 1:10,000), StarBright Blue 700 Goat Anti-Rabbit IgG (Bio-509 Rad; #12004161; 1:10,000), and StarBright Blue 520 Goat Anti-Rabbit IgG (Bio-Rad; #12005869; 510 1:10,000).

Staining:

Article Title: Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia
Article Snippet: .. Tissues were stained with goat anti-mouse CD31 antibody (R&D Systems, diluted 1:100 in 1% BSA Triton-X 0.3% solution) overnight at 4 °C, followed by incubation with Alexa FluorTM 568-conjugated chicken anti-goat antibody (Invitrogen - Thermo Fisher Scientific, diluted 1:1000 in 1x PBS), and Alexa FluorTM 488-conjugated rat anti-mouse αSMA antibody (eBioscience - Thermo Fisher Scientific, diluted 1:400 in 1x PBS) for 1 h at room temperature. .. The expression of CD31 and αSMA in the blood vessels was recorded by Leica TCS SP8 confocal microscope and the quantification of the mean length/diameter of blood vessels positive for these markers was analyzed using ImagePro Plus software version 7.0 (Media Cybernetics, Maryland, USA).

Article Title: Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia
Article Snippet: .. The nonspecific sites were blocked by incubation with the CAS-BlockTM histochemical reagent (InvitrogenTM - Thermo Fisher Scientific) for 10 min. To assess the presence of CD31-positive blood vessels, sections were stained with goat anti-mouse CD31 antibody (R&D Systems, diluted 1:100 in 1% Triton-X 0.3% BSA solution) overnight at 4 °C and subsequently incubated with chicken anti-goat antibody conjugated with Alexa FluorTM 568 (Invitrogen - Thermo Fisher Scientific, diluted 1:1000 in 1x PBS) for 1 h at room temperature. .. To assess the presence of arterioles, sections were stained with rat anti-mouse αSMA antibody conjugated with Alexa FluorTM 488 (eBioscience - Thermo Fisher Scientific, diluted 1:400 in 1x PBS) for 1 h at room temperature.

Incubation:

Article Title: Safety, Distribution, and Pharmacokinetics of Biodegradable P(AAm-co-MAA) Nanogels Following Systemic Administration in Mice.
Article Snippet: Systematic analysis of the fate of hydrogel nanoparticles after in vivo administration is essential for their clinical translation.. Biodegradable, disulfidecrosslinked synthetic nanogels are a promising platform for the delivery of therapeutic molecules, but their biodistribution and clearance profiles remain underexplored compared to other solid nanoparticles.. In this study, we investigated the safety, pharmacokinetics, tissue, and cellular distribution profiles of poly(acrylamidecomethacrylic acid) (P(AAmcoMAA)) nanogels following a single intravenous or intraperitoneal injection.

Article Title: Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia
Article Snippet: .. The nonspecific sites were blocked by incubation with the CAS-BlockTM histochemical reagent (InvitrogenTM - Thermo Fisher Scientific) for 10 min. To assess the presence of CD31-positive blood vessels, sections were stained with goat anti-mouse CD31 antibody (R&D Systems, diluted 1:100 in 1% Triton-X 0.3% BSA solution) overnight at 4 °C and subsequently incubated with chicken anti-goat antibody conjugated with Alexa FluorTM 568 (Invitrogen - Thermo Fisher Scientific, diluted 1:1000 in 1x PBS) for 1 h at room temperature. .. To assess the presence of arterioles, sections were stained with rat anti-mouse αSMA antibody conjugated with Alexa FluorTM 488 (eBioscience - Thermo Fisher Scientific, diluted 1:400 in 1x PBS) for 1 h at room temperature.

Article Title: A sexually dimorphic hepatic cycle of periportal VLDL generation and subsequent pericentral VLDLR-mediated re-uptake
Article Snippet: .. The primary antibody, goat anti-VLDLR (R&D Systems AF2258, diluted 1: 50 in 1% BSA in 1x PBS) was incubated 1 h at 37 °C. .. After incubation with goat Immpress HRP (Ready to use, Vector Laboratories), chromogenic revelation was performed with ChromoMap DAB kit.

other:

Article Title: A mouse-adapted Yezo virus model for antiviral testing in immunocompetent mice
Article Snippet: The commercial primary antibodies used in this study were as follows: anti-albumin (AF3329), anti-CD31 (AF3628), and anti-desmin (AF3844) (R&D Systems, USA); anti-F4/80 (ab320060) (Abcam, USA); anti-NLRP3 (15101S), anti-beta-Actin (4970S), and anti-GAPDH (2118S) (Cell Signaling Technology, USA).

Article Title: A mouse-adapted Yezo virus model for antiviral testing in immunocompetent mice.
Article Snippet: The commercial primary antibodies used in this study were as follows: anti-albumin (AF3329), anti-CD31 (AF3628), and anti-desmin (AF3844) (R&D Systems, USA); anti-F4/80 (ab320060) (Abcam, USA); anti-NLRP3 (15101S), anti-beta-Actin (4970S), and anti-GAPDH (2118S) (Cell Signaling Technology, USA).

Western Blot:

Article Title: Loss of the Endothelial Glycocalyx Component EMCN Leads to Glomerular Impairment
Article Snippet: RNase-Free DNase Set (#79254), RNeasy plus mini kit (#74136), and QIAamp Fast DNA Tissue Kit (#51404) were purchased from Qiagen. .. Immunoblots were probed with rat anti-mouse EMCN (1:1,000, Abcam, #ab106100), mouse anti-tubulin (1:2,000, Cell Signaling, #3873S), mouse anti-β-actin (1:2,000, Cell Signaling, #3700S), goat anti-albumin (1:1,000, R&D Systems, #AF3329-SP), or rabbit anti-podocin (1:1,000, Sigma, #P0372). .. Paraffin sections of kidneys were blocked with 5% donkey or goat serum in PBS-T (0.1% Tween-20) and then stained with rat antimouse EMCN (1:200, Abcam, #ab106100), rabbit anti-EGFP (1:300, Sigma, #SAB4701015), mouse anti-CD31 (1:200, Cell Signaling, #3528S), mouse anti-CD34 (1:200, Santa Cruz, #sc-74499), rat anti-CD45 (1:100, Abcam, #ab23910), goat antialbumin (1:300, R&D Systems, #AF3329-SP), rabbit anti-podocin (1:200, Thermo Fisher, #PA5-79757), and goat anti-smooth muscle actin (1:100, Thermo Fisher, #PA518292).

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